Coding

Part:BBa_I725000:Design

Designed by: Mike Deyholos   Group: iGEM07_Alberta   (2007-06-04)


Ca_20_21 coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 163
    Illegal AgeI site found at 294
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for E. coli expression using AccelerysGene.


Source

One of three potential genes from the Clostridium acetobutylicum genome putatively encoding this enzymatic function. Two of these are located on the plasmid, and one of these plasmid-localized genes was used to design this sequence.

References